An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew
Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), reduces wheat yields and grain quality, thus posing a significant threat to global food security. Wild relatives of wheat serve as valuable resources for resistance to powdery mildew. Here, the powdery mildew resistance gene Pm6Sl is cloned from the wild wheat species Aegilops longissima. It encodes a nucleotide-binding leucine-rich repeat (NLR) protein featuring a CC-BED module formed by a zinc finger BED (Znf-BED) domain integrated into the coiled-coil (CC) domain. The function of Pm6Sl is validated via mutagenesis, gene silencing, and transgenic assays. In addition, we develop a resistant germplasm harbouring Pm6Sl in a very small segment with no linkage drag along with the diagnostic gene marker pm6sl-1 to facilitate Pm6Sl deployment in wheat breeding programs. The cloning of Pm6Sl, a resistance gene with BED-NLR architecture, will increase our understanding of the molecular mechanisms underlying BED-NLR-mediated resistance to various pathogens.
Authors
- Guihong Yin (ORCID: https://orcid.org/0000-0002-3604-8423)
- Xiubin Tian
- Yusheng Zhao (ORCID: https://orcid.org/0000-0002-5893-504X)
- Wenxuan Liu (ORCID: https://orcid.org/0000-0003-1227-0991)
- Xuexue Chen (ORCID: https://orcid.org/0009-0003-0941-4939)
- Sunish K. Sehgal (ORCID: https://orcid.org/0000-0001-5644-7547)
- Huanhuan Li (ORCID: https://orcid.org/0009-0007-5808-1965)
- Zhibin Zhang (ORCID: https://orcid.org/0000-0003-1349-2713)
- Liwei Zhang (ORCID: https://orcid.org/0000-0001-5042-1960)
- Liu B (ORCID: https://orcid.org/0000-0001-5481-1675)
- Yue Zhao (ORCID: https://orcid.org/0000-0001-9351-7559)
- Chao Ma (ORCID: https://orcid.org/0009-0000-5691-8118)
- Qianwen Liu (ORCID: https://orcid.org/0000-0001-8020-7957)
- Hongfei Yan
- Shiwei Wang (ORCID: https://orcid.org/0000-0003-0836-0337)
- Aizhong Cao
- Zhenjie Dong
- Cheng Liu
- Shuyang Ma
Institutions
- Hebei Agricultural University (CN)
- Nanjing Agricultural University (CN)
- South Dakota State University (US)
- Northeast Normal University (CN)
- Chinese Academy of Sciences (CN)
- Shandong Academy of Agricultural Sciences (CN)
- Institute of Genetics and Developmental Biology (CN)
- University of Chinese Academy of Sciences (CN)
- Henan Agricultural University (CN)
- China Agricultural University (CN)
Publication Details
- Journal
- Nature Communications
- Published
- 2024-09-27
- DOI
- https://doi.org/10.1038/s41467-024-52670-2
- Citations
- 24
- Primary Topic
- Wheat and Barley Genetics and Pathology
- Type
- article
- Field-Weighted Citation Impact
- 14.39
Funders
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- Division of Graduate Education