INVESTIGATION ANTIOXIDANT, ANTIMICROBIAL EFFECT AND PHENOLIC COMPOUNDS OF Alchemilla holotrichia EXTRACTS IN DIFFERENT POLARITIES

In this study, different polarity extracts of Alchemilla holotrichia were obtained with hexane, dichloromethane, ethyl acetate, methanol and water. Then, total antioxidant activity, DPPH free radical scavenging activity, metal chelating capacity, total reducing power, hydrogen peroxide scavenging, hydroxy radical scavenging activity of these extracts were determined.Total phenol content 67.63± 0.88 GAE/g extract in the highest ethyl acetate extract, total flavonoid content 14.82±1.02–14.82±0.03 mg CE/g extract in the highest ethyl acetate and dichloromethane extract, phenolic acid content 0.72±0.21 mg SAE /g extract in the highest-hexane content Determination of Antimicrobial activity was used agar well diffusion method and the agar disk diffusion method. These methods was tested on Bacillus subtilis ATCC 6633, Escherichia coli ATCC25922, Staphylococcus aureus ATCC 29213, Candida albicans, Klebsiella pneumoniae EMCS, Saccharomyces cerevisiae. The ethyl acetate extract of Alchemilla holotrichia has emerged the highest activity. The highest antimibacterial effect on Escherichia coli (17 mm) in the following, Klebsiella pneumoniae (15 mm) has emerged. Bacillus subtilis was determined as the most resistant microorganism (8 mm). Alchemilla holotrichia showed both antioxidant and antimicrobial activity best in ethyl acetate extract. In the study, it was observed that ethyl acetate, which is a partially polar solvent, dissolves aromatic substances in the aerial part of the plant better than non-polar solvents such as n-hexane and diethyl ether.