Undiagnosed West Nile virus lineage 2d infection in a febrile patient from South-west Uganda, 2018
We report the retrospective identification and subsequent recovery of a near-complete West Nile Virus lineage 2 genomes from a hospitalized patient with acute febrile illness in Uganda, using a combination of degenerate primer polymerase chain reaction screening and a novel 1200bp nanopore-based whole-genome amplicon sequencing scheme. This represents the first West Nile virus genome to be recovered from a human in Uganda since its discovery in 1937. Basic molecular rather than serological surveillance methods could be more widely deployed in the region to better diagnose febrile infections.
Authors
- C. Patrick McClure (ORCID: https://orcid.org/0000-0002-1710-1049)
- Theocharis Tsoleridis (ORCID: https://orcid.org/0000-0002-9685-6318)
- John Kayiwa (ORCID: https://orcid.org/0000-0002-1840-1801)
- Irene Ataliba
- Julius J. Lutwama (ORCID: https://orcid.org/0000-0002-3210-722X)
- Joseph Chappell (ORCID: https://orcid.org/0000-0002-8205-4242)
- Stuart Astbury (ORCID: https://orcid.org/0000-0002-1919-3952)
- Annet Martha Nankya
- Timothy Byaruhanga (ORCID: https://orcid.org/0000-0003-2024-9172)
- Jonathan K. Ball (ORCID: https://orcid.org/0000-0001-5773-8733)
- Jack D. Hill
Institutions
- The Pirbright Institute (GB)
- Nottingham University Hospitals NHS Trust (GB)
- University of Nottingham (GB)
- Animal and Plant Health Agency (GB)
- Liverpool School of Tropical Medicine (GB)
- NIHR Nottingham Digestive Diseases Biomedical Research Unit (GB)
- Nottingham Biomedical Research Centre (GB)
- Uganda Virus Research Institute (UG)
Publication Details
- Journal
- IJID Regions
- Published
- 2024-09-26
- DOI
- https://doi.org/10.1016/j.ijregi.2024.100462
- Citations
- 1
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 0.54
Funders
- Medical Research Council