Cross-species single-cell spatial transcriptomic atlases of the cerebellar cortex

The molecular and cellular organization of the primate cerebellum remains poorly characterized. We obtained single-cell spatial transcriptomic atlases of macaque, marmoset, and mouse cerebella and identified primate-specific cell subtypes, including Purkinje cells and molecular-layer interneurons, that show different expression of the glutamate ionotropic receptor Delta type subunit 2 (<i>GRID2</i>) gene. Distinct gene expression profiles were found in anterior, posterior, and vestibular regions in all species, whereas region-selective gene expression was predominantly observed in the granular layer of primates and in the Purkinje layer of mice. Gene expression gradients in the cerebellar cortex matched well with functional connectivity gradients revealed with awake functional magnetic resonance imaging, with more lobule-specific differences between primates and mice than between two primate species. These comprehensive atlases and comparative analyses provide the basis for understanding cerebellar evolution and function.

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Journal
Science
Published
2024-09-26
DOI
https://doi.org/10.1126/science.ado3927
Citations
43
Primary Topic
Single-cell and spatial transcriptomics
Type
article
Field-Weighted Citation Impact
10.05

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article

Cross-species single-cell spatial transcriptomic atlases of the cerebellar cortex

Zihan Wu, Wei Wu, Shijie Hao, Xiaojia Zhu et al.
43 citations
Science
Single-cell and spatial transcriptomics
10.05
article

Cross-species single-cell spatial transcriptomic atlases of the cerebellar cortex

Zihan Wu, Wei Wu, Shijie Hao, Xiaojia Zhu, Shiping Liu, Zhenkun Zhuang, Cirong Liu, Zhiyong Liu, Qian Yu, Chengyu Li, Shenglong Li, Yafeng Zhan, Xinxiang Song, Suhong Sun, Chuanyu Liu, Longqi Liu, Mu‐ming Poo, Chris I. De Zeeuw, Guangling Wang, Yue Yuan, Jiabing Liu, Yan-Gang Sun, Mengni Chen, Huanhuan Li, Huanlin Liu, Zhiming Shen, Zhi Rui Huang, Elize D. Haasdijk, Luman Cui, Lei Han, Aojun Ye, Yu Chen, Yanqing Zhong, Yingjie An, Qinwen Chai, Ying Xia, Zhifeng Wang, Z.-H. Wang, Yuhui Dong, Ying Lei, S. D. Li, Nini Yuan, Shiyong Guo, Tao Yang, Zhifeng Liang, Chao Li, He Wang, Ruiqi Wang, Jing Chen, Zhen Liu, Ling Li, Xun Xu, Yan Wu, Jianhua Yao, Shiwen Wang, Jingjing Song, H.-C. Liu, Lijing Zhu, Qianqian Yang, Xiumei Lin, Youning Lin, Ruiyi Zhang, Jiao Fang, Yuanfang Xu, Yumo Song, Yun Zhao, Ming-Li Wang, Shuzhen Zhang, Haibo Zhou, Liqin Gu, Mengnan Cheng, Xing Tan, Wensi Du, Tingting Lai, Haotian Yan, Jie He, Wanqiu Zhou, Yang Wang, Mingyuan Zheng, Mengdi Cao, Chang Liu
article en
43 citations

Abstract

The molecular and cellular organization of the primate cerebellum remains poorly characterized. We obtained single-cell spatial transcriptomic atlases of macaque, marmoset, and mouse cerebella and identified primate-specific cell subtypes, including Purkinje cells and molecular-layer interneurons, that show different expression of the glutamate ionotropic receptor Delta type subunit 2 (<i>GRID2</i>) gene. Distinct gene expression profiles were found in anterior, posterior, and vestibular regions in all species, whereas region-selective gene expression was predominantly observed in the granular layer of primates and in the Purkinje layer of mice. Gene expression gradients in the cerebellar cortex matched well with functional connectivity gradients revealed with awake functional magnetic resonance imaging, with more lobule-specific differences between primates and mice than between two primate species. These comprehensive atlases and comparative analyses provide the basis for understanding cerebellar evolution and function.

ScienceVol. 385(6716)
BGI Group (China) (CN), Royal Netherlands Academy of Arts and Sciences (NL), Shanxi Medical University (CN), Kunming Institute of Zoology (CN), Chinese Academy of Sciences (CN), Tencent (China) (CN), Center for Excellence in Brain Science and Intelligence Technology (CN), China National GeneBank (CN), Shanghai Center for Brain Science and Brain-Inspired Technology (CN), University of Chinese Academy of Sciences (CN), BGI Research (CN), South China University of Technology (CN), Erasmus University Rotterdam (NL)
National Natural Science Foundation of China, Nederlandse Organisatie voor Wetenschappelijk Onderzoek, Youth Innovation Promotion Association of the Chinese Academy of Sciences, Youth Innovation Promotion Association
Life in Land
Openalex Percentile: Top 1%
Single-cell and spatial transcriptomics
10.05
DOIOpen Access PDFOpenAlex
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