Evaluation of the Anticarcinogenic Effect of White Radish Extract (Raphanus sativus var. Longipinnatus) on In Vitro Ehrlich Ascites Tumor Cells

In this research, it was aimed to research the time and different dose effects of white radish extract (Raphanus sativus var. Longipinnatus) on Ehrlich Ascites Tumor (EAT) cell lines. EAT cell line was used in the study. EAT cells were treated white radish extract (Raphanus sativus var. Longipinnatus) at 37°C and 5% CO2 for differing periods (24 and 48 hours) and doses (100-200 and 300 μg/ml white radish extract). At the end of the incubation duration, Argyrophilic nucleolar organizing region (AgNOR) protein condition of EAT cells were investigated.It was determined among the control and 300 μg/ml Raphanus sativus extract group the significant differences for mean AgNOR number and TAA/NA in 48 hours period. It was detected also between the 100 and 300 μg/ml Raphanus sativus extract groups for AgNOR number and TAA/NA in 48 hours incubation. This study demonstrated that Raphanus sativus had a important role against cancer cells. Also, both AgNOR values mit be used as biomarkers for identification of the most true therapeutic dose option for cancer and it has been shown that suitable ingestion of Raphanus sativus can be effective in avoid cancer development and slowing its spreading.