Studies on the Extraction of Guarana Peel Polysaccharides and its Effect on Blood Glucose Lipids in Mice

Objective: To study the extraction of Guarana peel polysaccharides (GPP) by two different methods and to investigate the effect of extracted polysaccharides on haemoglycaemia and lipids in mice. Methods: The polysaccharides were extracted by aqueous alcoholic precipitation with two different solvents, methanol/ethanol mixed solvent or 95% ethanol and the mice were fed and tested for changes in endogenous related substances, respectively. Results: The concentration of polysaccharide extracted by methanol/ethanol mixed solvent was 116.367 μg/mL, while the concentration of polysaccharide extracted by 95% ethanol was 101.465 μg/mL. The polysaccharide extracted by the former method did not significantly affect the blood glucose level of the mice, while the latter method did not significantly affect the lipid level of the mice. Conclusion: Methanol/ethanol mixed solvent extraction of Guarana peel polysaccharides had higher extraction rate and more significant effect on blood glucose control in mice, while 95% ethanol extraction of Guarana peel polysaccharides had more significant effect on lipid control in mice.