Research on depth enhancement of fluorescence imaging based on adaptive optics

Fluorescence imaging is one of the basic research of biological microscope imaging, and improving the imaging depth of biological microscopes has always been a research hotspot . One of the factors affecting the depth is that the excitation light will be absorbed and scattered by the tissue and cannot be focused on the target fluorescent area. Therefore, this study uses an adaptive optics scheme to correct the wavefront phase of the excitation light, and uses the intensity of fluorescence as a quantitative parameter. The results show that the research scheme in this paper has a good effect on improving the quality of deep imaging.