CHROMATOGRAPHIC SEPARATION OF HYDROPHILIC ORGANOPHOSPHATES ON A POROUS GRAPHITIZED CARBON SORBENT HYPERCARB USING AN AQUEOUS SOLUTION OF FORMIC ACID AS A MOBILE PHASE

The possibility of elution of hydrophilic organophosphorus substances using the concentration gradient of formic acid in an aqueous mobile phase on a porous graphitized carbon sorbent Hypercarb has been studied. The analytes were detected using a mono quadrupole mass spectrometer. To study the retention of analytes, the composition of the mobile phase was varied before injection of the sample solution. It is shown that the effect of stepwise gradient elution on the retention of analytes is primarily due to the state of the sorbent surface, rather than the elution ability of the phases, and that the displacing “quasi-ion exchange” mechanism of retention of analytes is the most likely.