Construction of HEK293T cell line stably expressing TRPM2 channel based on PiggyBac transposition system and its application in drug screening for cerebral ischemia and other diseases
A stable TRPM2 gene expressing cell line has been successfully established using PiggyBac gene editing in this study. TRPM2 channel inhibitors were screened through calcium imaging and patch clamp techniques, and an inhibitor compound 6 was identified. This compound can alleviate cell damage after OGD/R by reducing cellular ROS levels and has a protective effect against cerebral ischemia-reperfusion injury in mice.
Authors
- Kaiyue Ying
- Xingyu Liu (ORCID: https://orcid.org/0000-0002-9649-189X)
- Luo Yan-ping
- Ning Hua (ORCID: https://orcid.org/0009-0004-6545-7965)
- Min Liu (ORCID: https://orcid.org/0000-0002-5059-3743)
- Wei Yang (ORCID: https://orcid.org/0009-0005-8713-6262)
Institutions
- Zhejiang University (CN)
Publication Details
- Journal
- Journal of Zhejiang University (Medical Sciences)
- Published
- 2024-09-26
- DOI
- https://doi.org/10.3724/zdxbyxb-2024-0257
- Primary Topic
- Ion Channels and Receptors
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China