Protocol for a non-surgical model of perineural invasion for assessing neural drivers of cancer aggressiveness in mice
Perineural invasion (PNI) is a significant risk factor for cancer recurrence and metastasis; however, its mechanisms relating to cancer aggressiveness remain poorly understood. Here, we present a protocol for a non-surgical model of PNI in mice using a neurotropic melanoma cell line that migrates from the skin to the sciatic nerve. We describe the steps for cell culture and injection, tumor burden measurements, mouse euthanasia, and tissue dissection. We then detail procedures for sample cross-section and confocal imaging.
Authors
- David Y. Chen (ORCID: https://orcid.org/0000-0002-3681-6576)
- Michael Slade Stratton (ORCID: https://orcid.org/0000-0002-7069-3995)
- Felipe A. Pinho‐Ribeiro (ORCID: https://orcid.org/0000-0003-4264-9839)
- Telma Saraiva‐Santos (ORCID: https://orcid.org/0000-0003-3639-6441)
- Tiago H. Zaninelli (ORCID: https://orcid.org/0000-0001-7233-477X)
- Brielle H Patlin (ORCID: https://orcid.org/0000-0001-7130-7665)
Institutions
- Washington University in St. Louis (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-09-26
- DOI
- https://doi.org/10.1016/j.xpro.2024.103345
- Primary Topic
- Cancer, Stress, Anesthesia, and Immune Response
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Silicon Valley Community Foundation
- Elsa U. Pardee Foundation
- Institute of Clinical and Translational Sciences
- Chan Zuckerberg Initiative
- National Institutes of Health
- Washington University School of Medicine in St. Louis
- National Cancer Institute