Ameliorative Effect of Hydrocotyle Verticillata and Laportea Aestuans Leave Extracts on Hematological, Biochemical and Histopathological Indices on 7, 12-dimethylbenz (α) Anthracene (DMBA) Induced-Leukemia in Rats

This study evaluated the ameliorative effect of Hydrocotyle verticillata (H.v) and Laportea aestuans (L.a) leaves extracts on hematological, biochemical and histopathological indices on 7,12-dimethylbenz(α) anthracene (DMBA) induced-leukemia in wistar rats. Leukemia was induced in rats using standard method. Both extracts were evaluated for their effects on hematological, biochemical and histopathological indices using standard methods. The hematological indices showed a decrease in packed cell volume (21.83±0.85%) and hemoglobin concentrations (7.28±0.28g/dl) with an increase in Total white blood cells (30.08±0.51x109/L) in the negative control group. Treatment with the extracts significantly (p<0.05) increased the Hb, and PCV with a decrease in the TWBC levels in all treated groups. Liver enzymes of the standard drug and treated groups showed a significant (p<0.05) decrease when compared to the negative control (group 3) for both extracts. Urea (10.17±0.25mmol/l), creatinine (1.98±0.77mg/dl) hepatic enzymes levels reduced significantly (p<0.05) while Oxidative stress biomarker levels significantly (p<0.05) increased in the standard drug and treated groups when compared with the negative control. Histopathological examination showed strong hepato-renal protective effects. Hydrocotyle verticillata and Laportea aestuans have shown to possess hepato-renal protective effect and modulated hematological indices in induced-leukemia.