Molecular diagnosis of human papilloma virus (HPV) genotypes in women with genital warts in Mosul / Iraq

Human Papillomavirus (HPV) belongs to the Papillomaviridae family and can contribute to Cervical Cancer (CC). The present study aimed to investigate the genotypic distribution and prevalence of HPV using Phosphate Buffered Saline (PBS) embedded tissue, a cervical swab and a cervical brush in Viral transport medium (VTM) from women with different genital warts with no other disease in Mosul City/ Iraq. A total of 150 samples collected from women with genital warts infections were analyzed. A sequencing analysis of the amplified HPV L1 gene was made and compared with that of the National Center for Biotechnology Information (NCBI). The existence of HPV nucleic acid DNA was examined in all samples by Polymerase Chain Reaction (PCR) using MY09/MY11 and GP+5/GP+6 primers, and then HPV genotypes were identified in Macrogen (Macrogen Co., Seoul, Korea) BioEdit (version 7.2.5.0). From 150 (cervical swab, brush, and tissue) patients with genital warts, the distribution of HPV genotype frequency in females with vaginal warts was 127 patients (84.6%), 10 patients (8%) with High-Risk (HR-HPV) serotypes (4%) of each HPV (16 and 45), while the rest 117 patients (92%) with Low-Risk (LR-HPV) included 60.5% HPV11, 20.5% HPV6 and 11.% HPV10. For the first time in Mosul, Iraq, the study used PBS-embedded tissue from patients with various anogenital lesions to examine the prevalence and genotypic distribution of HPV.