Advancing SARS‐CoV‐2 Variant Detection with High Affinity Monoclonal Antibodies and Plasmonic Nanostructure
Abstract A nanoplasmonic biosensor for the precise detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) by leveraging advances in nanostructured sensing materials and highly selective monoclonal antibodies is presented. The sensor integrates plasmonic Au nanostructures optimized for surface‐enhanced Raman scattering (SERS), enhancing sensitivity through unique light interactions at the nanoscale. Coupled with exclusive antibodies that specifically target SARS‐CoV‐2 and its evolving variants, this sensor demonstrates remarkable selectivity and versatility. Validated with 270 clinical samples, it demonstrates a sensitivity of 98.9% and specificity of 100%. More importantly, the virus in nasopharyngeal swab samples collected over 3 years, marking the long‐term, large‐scale clinical validation of the nanoplasmonic biosensor for SARS‐CoV‐2 is been successfully detected. Furthermore, the integration of this sensor with face masks enables the detection of airborne SARS‐CoV‐2, highlighting its potential for routine management of coronavirus disease 2019 (COVID‐19).
Authors
- Joo Hoon Lee (ORCID: https://orcid.org/0000-0001-8010-3605)
- Eun‐Kyung Lim (ORCID: https://orcid.org/0000-0003-2793-3700)
- Sun‐Joo Kim (ORCID: https://orcid.org/0000-0001-8099-8891)
- Juyeon Jung (ORCID: https://orcid.org/0000-0002-1969-321X)
- Yongwon Jung (ORCID: https://orcid.org/0000-0002-1034-9797)
- Jea Sung Ryu
- Hyung‐Jun Kwon (ORCID: https://orcid.org/0000-0002-6429-8075)
- Ho Sang Jung (ORCID: https://orcid.org/0000-0003-1776-1802)
- Hyowon Jang (ORCID: https://orcid.org/0000-0001-5129-6719)
- Sung‐Gyu Park (ORCID: https://orcid.org/0000-0002-0580-3211)
- Taejoon Kang (ORCID: https://orcid.org/0000-0002-5387-6458)
- Soo Hyun Lee (ORCID: https://orcid.org/0000-0002-6690-741X)
- Hye-Ran Kim (ORCID: https://orcid.org/0000-0002-9399-0503)
- Hyunju Kang
- Pei Li (ORCID: https://orcid.org/0009-0006-6617-1824)
Institutions
- Korea Advanced Institute of Science and Technology (KR)
- Gyeongsang National University Hospital (KR)
- Korea Institute of Materials Science (KR)
- Korea Research Institute of Bioscience and Biotechnology (KR)
- Sungkyunkwan University (KR)
- Korea University of Science and Technology (KR)
Publication Details
- Journal
- Advanced Functional Materials
- Published
- 2024-09-16
- DOI
- https://doi.org/10.1002/adfm.202405340
- Citations
- 9
- Primary Topic
- Biosensors and Analytical Detection
- Type
- article
- Field-Weighted Citation Impact
- 1.66
Funders
- Ministry of Trade, Industry and Energy
- Ministry of Agriculture, Food and Rural Affairs
- Korea Research Institute of Bioscience and Biotechnology
- Ministry of Science and ICT, South Korea
- Korea Environmental Industry and Technology Institute
- Korea Evaluation Institute of Industrial Technology