Assessing the Toxic Effects of Topik80ec on Rabbit Testicular Morphometry and Histology

This study aimed to investigate the toxic impact of Topik80ec on rabbit testes through morphometric and histological analyses. Twenty-four adult male domestic rabbits (Oryctolagus cuniculus) were used, where three experimental groups, G1, G2, and G3, were assigned and provided escalating dosages of Topik80 EC for a duration of four weeks, while one group served as the control group and received distilled water. The morphometric characteristics, including weight, length, breadth, and thickness, of the testicles were evaluated. Histological examinations were performed on the testicular specimens, which were subjected to staining procedures using the Hematoxylin and Eosin (H&E) technique, as well as Azan's trichrome staining method. The results revealed a significant decline (p<0.05) in weight, length, and width in the treated groups compared to the control group, notably in group 3. Furthermore, significant alterations in the structural composition and tissue pathology of the testes in the rabbits subjected to treatment were detected compared to the control group, characterized by the degeneration of seminiferous tubules, as evidenced by the presence of atrophy, deformation, uneven epithelium, and a reduction in germinal cells, with an excessive build-up of collagen fiber within the testicular interstitial tissues resulting in the development of testicular interstitial fibrosis. These changes were particularly noticeable in the lower counts of spermatozoa at higher dosages (G3). The experimental compound Topik80 EC exhibited considerable toxicity towards the testes of male domestic rabbits, resulting in a significant reduction in fertility due to profound structural and functional harm.