Sustainable Biocatalytic System for the Enzymatic Epoxidation of Waste Cooking Oil

The present study is integrated in a global effort to capitalize waste cooking oil (WCO) into versatile compounds by introducing an oxirane ring into the unsaturated carbon chain of fatty acid residues (the epoxidation of double bound). Therefore, an enzymatic method was set up for the epoxidation of artificially adulterated WCO (SFw) and WCO under real conditions (SFr) derived from sunflower biomass. Commercial lipase (Novozyme, NZ) was used as a biocatalyst for generating the peracid requested by the epoxidation pathway. Optimum experimental conditions (e.g., 1.5 wt% NZ, 1:1:0.5 = H₂O₂/double bonds/peracid precursor (molar ratio) and 12 h reaction time) allowed for the conversion of 90% of the SFw substrate into products with an oxirane ring. Octanoic acid was selected as the best peracid precursor. The versatility of the developed system was tested for olive, milk thistle, hemp and linseed oils as both fresh and WCO samples. The characterization of the oil samples before and after the enzymatic epoxidation allowed for the evaluation of the system performance. SFw/SFr exhibited a better susceptibility to enzymatic epoxidation. In addition, the reusability of the biocatalytic system was investigated. Furthermore, different strategies, such as biocatalyst coating and the addition of organic solvents/buffers were applied, limiting enzyme leaching, for the better recovery of the biocatalyst activity.