Formulation and Characterization of Arginine Nanoparticle for Topical Drug Delivery

The modification of tamarind seed polysaccharide has greatly expanded its application in the field of medicine.In this study, lowmolecular-weight chitosan was modified with arginine (Arg) by a simple method.The identification by the Fourier transform infrared spectra (FTIR) showed that Arg was successfully covalently attached to the TSP.Interestingly, Arg-TSP was identified as nanoparticles by atomic force microscopy (AFM) and Scanning electron microscopy (SEM), whose particle size was 75.76 12.07 nm based on Dynamic Light Scattering (DLS) characterization.Then, whether the prepared Arg-CS nanoparticles could encapsulate and deliver siRNA safely was investigated.Arg-TSP was found to be able to encapsulate siRNAs in vitro via electrostatic interaction with siRNA; the Arg-TSP/siRNA complex was safe for L1210 leukemia cells.