Phytochemical Screening Using LC-MS to Study Antioxidant and Toxicity Potential of Methanolic Extracts of Atraphaxis pyrifolia Bunge

<i>Atraphaxis pyrifolia</i>, a native medicinal plant of Central Asia, has a long history of traditional medicinal use; however, scientific research on its phytochemical and biological properties remains scarce. This paper aims to elucidate its chemical profile and assess its pharmacological potential through a comprehensive investigation of the phytochemical composition of stems and leaves using Liquid Chromatography-Mass Spectrometry (LC-MS), in conjunction with the assessment of its antioxidant (DPPH and ABTS) and cytotoxicity test on <i>Artemia salina</i>. Predominantly, glycosylated flavonoids were detected in stems and leaves extracts, notably including 8-Acetoxy-3',4',5,5'-tetrahydroxy-7-methoxy-3-α-L-rhamno-pyranosyloxyflavone, pyrifolin, and dehydroxypyrifolin. While the latter compound is exclusive to <i>A. pyrifolia</i>, the former compounds serve as shared chemical markers with other <i>Atraphaxis</i> species. The methanolic extracts of <i>A. pyrifolia</i> leaves exhibited significant antioxidant capacity without toxicity against <i>Artemia salina</i>. This study contributes to current research through providing valuable insights into the chemical diversity and potential medicinal properties of this plant species.