Temperature Interference on ZIKV and CHIKV Cycles in Mosquitoes and Mammalian Cells
Temperature is a determining factor for the viral cycle. In this study, we investigate the effect of different temperatures on the cycles of two important arboviruses-Zika (ZIKV) and Chikungunya (CHIKV)-in Vero (mammalian) and C6/36 (mosquito) cells. We compare genome quantification to infectivity at 28 °C and 37 °C in both cell types. Virus-cell interaction was also examined by transmission electron microscopy, allowing the observation of phenomena such as virus-surfing and giant forms for CHIKV, as well as the the scarcity of ZIKV in C6/36 cells compared to its cycle in mammalian cells.
Authors
- Lúcio Ayres Caldas (ORCID: https://orcid.org/0000-0002-1187-557X)
- Wanderley de Souza (ORCID: https://orcid.org/0000-0002-1895-1299)
- Tiago Souza Salles (ORCID: https://orcid.org/0000-0002-5822-1426)
- Érica S. Martins-Duarte (ORCID: https://orcid.org/0000-0003-0925-6953)
- Monica Ferreira Moreira (ORCID: https://orcid.org/0000-0002-3720-415X)
- Renata Campos Azevedo (ORCID: https://orcid.org/0000-0001-6068-9020)
- Davis Ferreira (ORCID: https://orcid.org/0000-0001-9449-4862)
- Marcelo Damião Ferreira de Meneses
Institutions
- Universidade Federal de Minas Gerais (BR)
- Universidade Federal do Rio de Janeiro (BR)
- Duke Medical Center (US)
- National Institute of Science and Technology for Structural Biology and Bioimaging (BR)
- Duke University Hospital (US)
- Fundação Oswaldo Cruz (BR)
Publication Details
- Journal
- Pathogens
- Published
- 2024-09-21
- DOI
- https://doi.org/10.3390/pathogens13090814
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 0.00