The Arabidopsis U1 snRNP regulates mRNA 3′-end processing

The removal of introns by the spliceosome is a key gene regulatory mechanism in eukaryotes, with the U1 snRNP subunit playing a crucial role in the early stages of splicing. Studies in metazoans show that the U1 snRNP also conducts splicing-independent functions, but the lack of genetic tools and knowledge about U1 snRNP-associated proteins have limited the study of such splicing-independent functions in plants. Here we describe an RNA-centric approach that identified more than 200 proteins associated with the Arabidopsis U1 snRNP and revealed a tight link to mRNA cleavage and polyadenylation factors. Interestingly, we found that the U1 snRNP protects mRNAs against premature cleavage and polyadenylation within introns-a mechanism known as telescripting in metazoans-while also influencing alternative polyadenylation site selection in 3'-UTRs. Overall, our work provides a comprehensive view of U1 snRNP interactors and reveals novel functions in regulating mRNA 3'-end processing in Arabidopsis, laying the groundwork for understanding non-canonical functions of plant U1 snRNPs.

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Publication Details

Journal
Nature Plants
Published
2024-09-23
DOI
https://doi.org/10.1038/s41477-024-01796-8
Citations
12
Primary Topic
RNA Research and Splicing
Type
article
Field-Weighted Citation Impact
2.81

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article

The Arabidopsis U1 snRNP regulates mRNA 3′-end processing

Thomas Schmutzer, Irina Droste-Borel, S L Streicher, Gregor Rot et al.
12 citations
Nature Plants
RNA Research and Splicing
2.81
article

The Arabidopsis U1 snRNP regulates mRNA 3′-end processing

Thomas Schmutzer, Irina Droste-Borel, S L Streicher, Gregor Rot, Cornelius Schmidtke, Luise Nagel, M. Adler, Joachim Weber, Boris Maček, Anchilie G. Francisco‐Mangilet, Sascha Laubinger, Sandra Schüler, Eneza Yoeli Mjema, Monique Renneberg, Paula Heilmann, Angie Herold
article en
12 citations

Abstract

The removal of introns by the spliceosome is a key gene regulatory mechanism in eukaryotes, with the U1 snRNP subunit playing a crucial role in the early stages of splicing. Studies in metazoans show that the U1 snRNP also conducts splicing-independent functions, but the lack of genetic tools and knowledge about U1 snRNP-associated proteins have limited the study of such splicing-independent functions in plants. Here we describe an RNA-centric approach that identified more than 200 proteins associated with the Arabidopsis U1 snRNP and revealed a tight link to mRNA cleavage and polyadenylation factors. Interestingly, we found that the U1 snRNP protects mRNAs against premature cleavage and polyadenylation within introns-a mechanism known as telescripting in metazoans-while also influencing alternative polyadenylation site selection in 3'-UTRs. Overall, our work provides a comprehensive view of U1 snRNP interactors and reveals novel functions in regulating mRNA 3'-end processing in Arabidopsis, laying the groundwork for understanding non-canonical functions of plant U1 snRNPs.

Nature PlantsVol. 10(10)
SIB Swiss Institute of Bioinformatics (CH), Carl von Ossietzky Universität Oldenburg (DE), University of Zurich (CH), Max Planck Institute for Plant Breeding Research (DE), Martin Luther University Halle-Wittenberg (DE), University of Tübingen (DE)
Deutsche Forschungsgemeinschaft
Openalex Percentile: Top 9%
RNA Research and Splicing
2.81
DOIOpen Access PDFOpenAlex
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