Simultaneous Determination of Cefdinir and Clavulanic Acid in Tablets by Using HighPerformance Liquid Chromatographic Methods

Background: A novel, rapid, and easy liquid chromatographic method was developed and validated for the simultaneous measurement of clavulanic acid and cefdinir in tablets. Methods: Reverse phase C18 column was used for the chromatographic separation, isocratic application of methanol-20.0 mM phosphate buffer (pH 3) (35:65, v/v) was used as the mobile phase system. Results: For clavulanic acid and cefdinir, the linearity ranges were determined to be 7.5-35.0 μg/mL and 18.0-84.0 μg/mL, respectively. It was calculated that the limits of quantification and detection for cefdinir and clavulanic acid, respectively, were 1.91 and 0.63 μg/mL and 1.33 and 0.44 μg/mL. Clavulanic acid and cefdinir were found to have mean recoveries of 96.645-104.26% and 96.60-98.52%, respectively. The suggested techniques were effectively used to identify commercially available tablets, and the outcomes were statistically compared using the Student’s t and Fischer’s F tests pharmacopeia methods. It was discovered that, at 95% CIs, there is no discernible difference between the approaches’ mean values and SDs. Conclusion: The method that has been devised is easy to use, rapid, accurate, repeatable, and safe for routinely determining the simultaneous presence of clavulanic acid and cefdinir in tablets.Cite this article as: Alp, AR, Ertürk Toker, S, & Ergin Kızılçay, G. Simultaneous determination of cefdinir and clavulanic acid in tablets by using high-performance liquid chromatographic methods. Trends Pharm, 2024, 1, 17, doi: 10.5152/TrendsPharm.2024.24017.