Alveolar macrophage function is impaired following inhalation of berry e-cigarette vapor
In the lower respiratory tract, the alveolar spaces are divided from the bloodstream and the external environment by only a few microns of interstitial tissue. Alveolar macrophages (AMs) defend this delicate mucosal surface from invading infections by regularly patrolling the site. AMs have three behavior modalities to achieve this goal: extending cell protrusions to probe and sample surrounding areas, squeezing the whole cell body between alveoli, and patrolling by moving the cell body around each alveolus. In this study, we found Rho GTPase, cell division control protein 42 (CDC42) expression significantly decreased after berry-flavored e-cigarette (e-cig) exposure. This shifted AM behavior from squeezing to probing. Changes in AM behavior led to a reduction in the clearance of inhaled bacteria, <i>Pseudomonas aeruginosa</i>. These findings shed light on pathways involved in AM migration and highlight the harmful impact of e-cig vaping on AM function.
Authors
- Amelia Kulle (ORCID: https://orcid.org/0000-0003-2402-7534)
- Maziar Divangahi (ORCID: https://orcid.org/0000-0002-1254-6110)
- Julianna Blagih (ORCID: https://orcid.org/0000-0002-5010-2879)
- Daina Zofija Avizonis (ORCID: https://orcid.org/0000-0002-3843-255X)
- Ajitha Thanabalasuriar (ORCID: https://orcid.org/0000-0001-6281-3013)
- David Langlais (ORCID: https://orcid.org/0000-0003-4429-0110)
- Mathieu Mancini (ORCID: https://orcid.org/0000-0002-4981-7121)
- Ziyi Li (ORCID: https://orcid.org/0009-0008-8071-0737)
- Irah L. King (ORCID: https://orcid.org/0000-0001-6990-7231)
- Erika Dianne Penz (ORCID: https://orcid.org/0000-0003-2610-8450)
- Marcel A. Behr (ORCID: https://orcid.org/0000-0003-0402-4467)
- Carolyn J. Baglole (ORCID: https://orcid.org/0000-0002-3107-0437)
- Antoine Dufour (ORCID: https://orcid.org/0000-0002-3429-4188)
- Daniel Young
- Marc Groleau
- Ashley Kwak
- Jing Wang (ORCID: https://orcid.org/0000-0003-3063-3733)
Institutions
- University of Calgary (CA)
- Shanghai Jiao Tong University (CN)
- McGill University Health Centre (CA)
- University of Saskatchewan (CA)
- Institute of Infection and Immunity (CA)
- McGill University (CA)
- Université de Montréal (CA)
Publication Details
- Journal
- Proceedings of the National Academy of Sciences
- Published
- 2024-09-23
- DOI
- https://doi.org/10.1073/pnas.2406294121
- Citations
- 12
- Primary Topic
- Neonatal Respiratory Health Research
- Type
- article
- Field-Weighted Citation Impact
- 5.03
Funders
- McGill University
- McGill University Health Centre
- Shanghai Jiao Tong University
- Canadian Institutes of Health Research
- School of Medicine, Shanghai Jiao Tong University