Effects of Different Photoperiods on the Transcriptome of the Ovary and Small White Follicles in Zhedong White Geese

Photoperiod can regulate the broodiness of geese and thus increase their egg-laying rate. The laying performance of geese is mainly determined by ovary and follicle development. To understand the effect of photoperiod on the ovary and small white follicles, sixteen 220-day-old healthy female Zhedong white geese were randomly divided into two groups for long photoperiods (15L:9D) and short photoperiods (9L:15D). The geese were euthanized after two months of feeding, and their ovaries and follicles were collected for transcriptome sequencing. RNA-seq analysis identified 187 and 448 differentially expressed genes in ovaries and small white follicles of different photoperiod groups, respectively. A long photoperiod promotes high expression of <i>SPP1</i>, <i>C6</i>, <i>MZB1</i>, <i>GP1BA,</i> and <i>FCGBP</i> genes in the ovaries, and increases the expression of <i>SPP1</i>, <i>ANGPTL5</i>, <i>ALPL</i>, <i>ZP1,</i> and <i>CHRNA4</i> genes in small white follicles. Functional enrichment analysis showed that photoperiod could affect respiratory system development, smooth muscle cell proliferation in ovaries, and extracellular matrix-related function in small white follicles. WGCNA revealed 31 gene modules, of which 2 were significantly associated with ovarian weight and 17 with the number of small white follicles. Our results provide a better understanding of the molecular regulation in the photoperiod affecting goose reproduction.