Assessment of twelve echovirus virus-neutralisation assays in Europe: recommendations for harmonisation of non-polio enterovirus sero-surveillance studies
Non-polio enteroviruses (NPEV) cause significant disease worldwide. Population-based sero-surveillance, by measuring antibodies against specific NPEV types, provides additional information on past circulation and the prediction for future upsurges. Virus neutralisation assays (VNA), the current method of choice for measuring NPEV type specific antibodies, are not entirely standardised. Via the European Non-Polio Enterovirus Network, we organised a VNA quality assessment in which twelve laboratories participated. We provided five echovirus (E) types (E1, E18, E30 G2, E30 G6 and E6) and intravenous immunoglobulins (IVIG) as a sample for the NPEV VNA quality assessment. Differences in VNA protocols and neutralising Ab (nAb) titres were found between the participating laboratories with geometric coefficients of variation ranging from 10.3-62.9 %. Mixed-effects regression analysis indicated a small but significant effect of type of cell line used. Harmonisation of cell line passage number, however, did not improve variation between laboratories. Calibration by making use of a reference sample, reduced variation between laboratories but differences in nAb titres remained higher than two log2 dilution steps. In conclusion, sero-surveillance data from different laboratories should be compared with caution and standardised protocols are needed.
Authors
- Petri Susi (ORCID: https://orcid.org/0000-0002-9245-3271)
- Karen Couderé (ORCID: https://orcid.org/0000-0002-6203-8499)
- Gerrit Koen
- Petra Rainetová (ORCID: https://orcid.org/0000-0001-7225-0795)
- Kimberley Benschop (ORCID: https://orcid.org/0000-0002-8328-0882)
- Hetty van Eijk
- Asya Stoyanova (ORCID: https://orcid.org/0000-0001-6219-8524)
- Dasja Pajkrt (ORCID: https://orcid.org/0000-0002-5232-8206)
- Vladimir Stevanović (ORCID: https://orcid.org/0000-0002-9572-8760)
- Marie‐Line Joffret (ORCID: https://orcid.org/0000-0002-5244-5429)
- Jean‐Luc Bailly (ORCID: https://orcid.org/0000-0002-9770-6450)
- Lubomira Nikolaeva–Glomb (ORCID: https://orcid.org/0000-0002-8008-2821)
- María Cabrerizo (ORCID: https://orcid.org/0000-0001-7054-5696)
- Sabine Diedrich (ORCID: https://orcid.org/0000-0002-3454-2647)
- Irena Tabain (ORCID: https://orcid.org/0000-0002-2518-522X)
- Željka Hruškar (ORCID: https://orcid.org/0000-0002-9078-1773)
- Maartje Kristensen (ORCID: https://orcid.org/0000-0003-2460-6958)
- Heli Harvala (ORCID: https://orcid.org/0000-0001-9154-4190)
- Sindy Böttcher (ORCID: https://orcid.org/0000-0002-7521-093X)
- Paula Palminha
- Eero Hietanen (ORCID: https://orcid.org/0000-0002-8548-3990)
- Jean‐Luc Murk (ORCID: https://orcid.org/0000-0003-3661-9035)
- Anda Băicuş (ORCID: https://orcid.org/0000-0002-4929-6493)
- Everlyn Kamau (ORCID: https://orcid.org/0000-0003-4285-2255)
- Maël Bessaud (ORCID: https://orcid.org/0000-0001-6758-9591)
- Katja C. Wolthers (ORCID: https://orcid.org/0000-0003-1617-7049)
- Irina Georgieva (ORCID: https://orcid.org/0000-0002-4814-4922)
- Isabelle Simon
Institutions
- Centre National de la Recherche Scientifique (FR)
- NHS Blood and Transplant (GB)
- University of Leeds (GB)
- University of Turku (FI)
- Institut Pasteur (FR)
- Université Clermont Auvergne (FR)
- Université Paris Cité (FR)
- Robert Koch Institute (DE)
- Instituto de Salud Carlos III (ES)
- European Nuclear Education Network (FR)
- University of Oxford (GB)
- Emma Kinderziekenhuis (NL)
- Hrvatski zavod za javno zdravstvo (HR)
- National Institute for Public Health and the Environment (NL)
- Croatian Veterinary Institute (HR)
- Laboratoire Microorganismes Génome et Environnement (FR)
- National Institute of Public Health (CZ)
- National Institute of Health Dr. Ricardo Jorge (PT)
- Elisabeth-TweeSteden Ziekenhuis (NL)
- Amsterdam University Medical Centers (NL)
- Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (ES)
- National Center of Infectious and Parasitic Diseases (BG)
- University of Amsterdam (NL)
Publication Details
- Journal
- Journal of General Virology
- Published
- 2024-09-23
- DOI
- https://doi.org/10.1099/jgv.0.001986
- Citations
- 3
- Primary Topic
- Viral Infections and Immunology Research
- Type
- article
- Field-Weighted Citation Impact
- 1.21
Funders
- Amsterdam University Medical Centers
- Instituto de Salud Carlos III
- Rijksinstituut voor Volksgezondheid en Milieu
- Centrum Infectieziektebestrijding, Rijksinstituut voor Volksgezondheid en Milieu