Using N-acylation as a disulfide lock simplifies chemical protein synthesis

Proportion (%) Equilibration time (min)Using N-acylation as a disulfide lock simplifies chemical protein synthesisTo compare the redox properties of SuCys peptides with their N-acylated derivatives, a 1:1 mixture of two model compounds was partially reduced using tris(2-carboxyethyl)phosphine (TCEP).To monitor the equilibration between the reduced and oxidised SutCys species present in solution, the reduced forms of SutCys were trapped by thiol alkylation with an excess of iodoacetamide prior to HPLC analysis.