Application of MALDI-TOF mass spectrometry for identification of Nocardia species
The Bruker mass spectrometer can accurately identify Nocardia species, albeit with some variations observed between different database versions. The MALDI Biotyper 3.1 (V09.0.0.0_8468) has limitations in identifying Nocardia brasiliensis, with some strains only identifiable to the genus level. MBT Compass 4.1 (V11.0.0.0_10833) effectively addresses this shortfall, improving species identification accuracy to 98.7%, and offering quick and reliable identification of Nocardia. Both database versions incorrectly identified the clinically less common Nocardia sputorum as Nocardia araoensis. For laboratories that have not upgraded their databases and are unable to achieve satisfactory identification results for Nocardia, employing the new and simplified extraction method can provide a degree of improvement in identification outcomes.
Authors
- Mei Kang (ORCID: https://orcid.org/0000-0002-6625-4834)
- Siying Wu (ORCID: https://orcid.org/0000-0003-0576-386X)
- Ying Tang (ORCID: https://orcid.org/0000-0003-4357-2343)
- Kaiwen Zhuang (ORCID: https://orcid.org/0000-0002-1376-6385)
- Yuling Xiao (ORCID: https://orcid.org/0000-0003-1237-941X)
- Quanfeng Liao
- Ya Liu (ORCID: https://orcid.org/0000-0001-9829-7567)
- Weili Zhang (ORCID: https://orcid.org/0000-0002-7956-7688)
- Nan Wu (ORCID: https://orcid.org/0000-0002-7207-2084)
- Jin Deng (ORCID: https://orcid.org/0000-0002-5218-9699)
Institutions
- Sichuan University (CN)
- West China Hospital of Sichuan University (CN)
- Kangmei Pharmaceutical (China) (CN)
- Shanghai Clinical Research Center (CN)
Publication Details
- Journal
- BMC Microbiology
- Published
- 2024-09-20
- DOI
- https://doi.org/10.1186/s12866-024-03483-2
- Citations
- 11
- Primary Topic
- Actinomycetales infections and treatment
- Type
- article
- Field-Weighted Citation Impact
- 17.32
Funders
- Sichuan University
- Sichuan Province Science and Technology Support Program
- West China Hospital, Sichuan University