Evaluation of Liver Antioxidants in an induced Oxidative Stress in Albino Rat Model

Background: Oxidative stress is a state of persistent imbalance between reactive oxygen species generation and ability of cellular antioxidant system to deactivate them.Objectives: The present case-control study aimed to evaluate antioxidants of liver in an induced oxidative stress by lipopolysaccharide (LPS) in an albino rat model at different time intervals (6 and 72 hours) and the correlations between the studied antioxidant indicators.Materials and methods: Ninety albino rats (male and female) were divided into two groups: 30 rats (control group) and 60 rats (test group)) were injected intraperitoneally (i.p) with saline and 60 rats (test group) injected i. p with 100 g/kg of LPS.Rats were sacrificed at intervals of 6 and 72 hours for evaluatingTumor necrosis factor ) TNF- ) , Myeloperoxidase (MPO), caspase-3, Cyclooxygenase-2 (COX-2), Interleukin-6 (IL-6), Malondialdehyde (MDA), Catalase, Glutathione peroxidase (GPx), Glutathione reductase (GR) and superoxide dismutase (SOD)in liver tissues using ELISA technique.Results: Our results revealed significant elevation in TNF, MPO, Caspase-3, COX-2, IL-6 and MDA levels, a significant reduction in catalase level and insignificant decrease in GPx, GR and SOD levels in both time intervals compared to the controls.There was significant difference in the indicator levels after 6 hours compared to 72 hour intervals, but it was insignificant for MPO, Caspase-3, GPx, GR and SOD.There were positively significant correlations for all indicators at 6 and 72-hours intervals except for MDA with GPx, GR and SOD, for MDA with catalase, GPx, GR and SOD.They were negatively significant at 6 and 72-hours intervals respectively; also, correlations were insignificant between catalase and GR after 6-hours interval and between TNF and catalase, MDA and MPO, IL-6 and MPO, GPx and SOD after 72-hours interval. Conclusion:The levels of the studied indicators were increased in LPS-induced oxidative stress up to 72 hours except for GPx, GR and SOD; catalase was decreased significantly at both time intervals.Upon comparing 6-hours to 72-hours intervals, significant difference was detected in all indicators except for MPO, Caspase-3, GPx, GR and SOD.Correlations between indicators were positively and negatively significant except at 6-hours interval for catalase and GR, at 72-hours interval for TNF and catalase, MDA and MPO, IL-6 and MPO, GPx and SOD after 72-hours interval, correlations were insignificant.