The splicing factor <scp>hnRNPL</scp> demonstrates conserved myocardial regulation across species and is altered in heart failure
Heart failure (HF) is highly prevalent. Mechanisms underlying HF remain incompletely understood. Splicing factors (SF), which control pre-mRNA alternative splicing, regulate cardiac structure and function. This study investigated regulation of the splicing factor heterogeneous nuclear ribonucleoprotein-L (hnRNPL) in the failing heart. hnRNPL protein increased in left ventricular tissue from mice with transaortic constriction-induced HF and from HF patients. In left ventricular tissue, hnRNPL was detected predominantly in nuclei. Knockdown of the hnRNPL homolog Smooth in Drosophila induced cardiomyopathy. Computational analysis of predicted mouse and human hnRNPL binding sites suggested hnRNPL-mediated alternative splicing of tropomyosin, which was confirmed in C2C12 myoblasts. These findings identify hnRNPL as a sensor of cardiac dysfunction and suggest that disturbances of hnRNPL affect alternative splicing in HF.
Authors
- Hannah R. Littel (ORCID: https://orcid.org/0009-0007-6399-788X)
- Ariel Mei (ORCID: https://orcid.org/0000-0002-0327-757X)
- Timothy D. Calamaras
- Mary C. Wallingford (ORCID: https://orcid.org/0000-0002-3682-156X)
- Mekala Gunasekaran
- Wan‐Ting Huang (ORCID: https://orcid.org/0000-0001-6442-3713)
- Isabelle Draper (ORCID: https://orcid.org/0000-0002-3888-1670)
- Suchita Pande (ORCID: https://orcid.org/0000-0002-1516-146X)
- Robert M. Blanton (ORCID: https://orcid.org/0000-0002-6837-2076)
- Vicky K. Yang (ORCID: https://orcid.org/0000-0002-1281-4250)
- Christine C. Bruels (ORCID: https://orcid.org/0000-0001-8366-3691)
- Peter B. Kang (ORCID: https://orcid.org/0000-0002-4270-7325)
- Richard H. Choe
- Ana Correia‐Branco (ORCID: https://orcid.org/0000-0001-5353-9665)
- Donna K. Slonim (ORCID: https://orcid.org/0000-0003-3357-437X)
- Audrey L. Daugherty (ORCID: https://orcid.org/0009-0005-0182-2238)
- Matthew J. Wolf (ORCID: https://orcid.org/0000-0001-8004-4852)
- Howard H. Chen (ORCID: https://orcid.org/0000-0002-5145-0584)
- Aaron Zou
- Natalya M. Wells
Institutions
- Tufts University (US)
- Tufts Medical Center (US)
- University of Minnesota (US)
- University of Virginia (US)
Publication Details
- Journal
- FEBS Letters
- Published
- 2024-09-19
- DOI
- https://doi.org/10.1002/1873-3468.15020
- Citations
- 1
- Primary Topic
- RNA Research and Splicing
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- Foundation for the National Institutes of Health
- American Heart Association
- National Institutes of Health