In-vitro antioxidant and antidiabetic activity of Crinum latifolium

The present study was done to investigate the In-Vitro antioxidant and antidiabetic activity of ethanolic extract of Crinum latifolium leaves. Phytochemicals of Crinum latifolium leaf extract was analysed by using qualitative methods. In-Vitro antioxidant and antidiabetic study were done by Hydrogen peroxide (H2O2) assay and Glucose uptake in Yeast cells method respectively. Phytochemical screening of ethanolic extract of leaves of Crinum latifolium revealed that it is rich in alkaloids, glycosides, tannins, flavonoids, gums and mucilage’s. The results for Hydrogen peroxide assay showed significant inhibitory potential in graded dose responsive against H2O2 free radicals at different concentrations of ascorbic acid (0.2 to 1.2 mg/ml). The standard ascorbic acid showed better inhibitory activity compared to sample, however 1.2 mg/ml ethanolic extract showed 71.94% inhibition and found to be equivalent to 76.82% of ascorbic acid. The results for Glucose uptake in Yeast cells method showed significant uptake of glucose across the plasma membrane of yeast cells at concentrations of (0.2 to 1.2 mg/ml) at 5mM glucose concentration. The standard Metronidazole showed better activity compared to sample, however 1.2 mg/ml ethanolic extract showed 83.07 % of glucose uptake and found to be equivalent to 87.98 % of Metronidazole. Overall, the present study results suggested that ethanolic extract of Crinum latifolium leaves has potential antioxidant and antidiabetic activities. However, further preclinical studies have to be done along with in vivo studies for confirming the results.