TWO-DIMENSIONAL SYNERGISTIC DYNAMICS OF BUFFER AND CALCIUM IN AN OBESE AND NORMAL HEPATOCYTE CELL

Cellular signaling is based on calcium ions (Ca[Formula: see text]), with proteins called buffers regulating their concentration. Previous research works have often treated buffer levels as constant but it is essential to acknowledge that buffers also undergo diffusion, causing fluctuations in their concentration within cells. Previous research works have overlooked the interconnected dynamics of calcium and buffers in cells. This research presents a novel two-dimensional framework integrating reaction–diffusion equations, governing calcium ions and buffers within both normal and obese hepatocyte cells. Additionally, it incorporates blip and puff phenomena which serve as significant sources influencing cellular signaling pathways. Cell’s physiological conditions are used to formulate boundary and initial conditions. This research delves into the repercussions of various parameters by employing finite element simulation, including the SERCA pump, inositol 1,4,5-triphosphate receptor (IP3R) and diffusion coefficient, on the spatio-temporal dynamics of calcium and buffers. Furthermore, it conducts a comparative examination of calcium dynamics between normal and obese hepatocyte cells. This model provides a heightened verisimilitude by encompassing the intertwined regulatory influences of calcium and buffers, distinguishing itself from prevailing models that treat these signaling mechanisms as isolated entities within hepatocyte cells.