ACUTE TOXICITY EVALUATION OF CURCUMA DOMESTICA VAHL. RHIZOME VCO CURCUMINOID EXTRACT

Objective: Recently, Curcuma domestica has been reported to have various pharmacological activities, such as antioxidant, anti-inflammatory and antifungal activities. The current study was conducted to evaluate the safety of C. domestica VCO Curcuminoid extract in short term and determine its curcumin content. Methods: Turmeric extract in VCO was prepared using a Microwave Assisted Extract (MAE) method, acute toxicity tests were carried out using 15 female mice divided into 5 preliminary test groups with 5, 50, 300, 1000, 2000 mg/KgBW doses. Furthermore, 10 female mice divided into 2 groups with 1000, 2000 mg/Kg BW doses as the main test. At the end of the experiments, the blood hematology, blood clinical biochemistry, and histopathology examination were performed. Results: Turmeric rhizome curcuminoid VCO extract at the dose of 2000 mg/Kg BW indicated signs of toxic symptoms, including diarrhea, changes in fur and skin and walking on the stomach. There are significant difference among the data of blood biochemistry and blood hematology of the CMC-Na group, with extract at the doses of 1000 mg/KgBW. Conclusion: It can be inferred that the turmeric rhizome VCO curcuminoid extract induces acute toxicity at a dose of 2000 mg/KgBW.