Toxicity of Yttrium-Doped Cerium Nanocomposite in <i>Drosophila Melanogaster</i>

Cerium oxide (CeO[Formula: see text] and yttrium oxide (Y 2 O[Formula: see text] nanoparticles possess interesting surface properties and interfacial interactions that make them attractive candidates for various applications. This study presents a comprehensive investigation into the synthesis and toxicity assessment of yttrium-doped cerium oxide (Ce-Y) nanocomposite using a combination of analytical techniques and biological assays. The nanocomposite was characterized using UV–Vis spectroscopy, X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), electron microscopy imaging, dynamic light scattering (DLS), and zeta potential measurements to elucidate their structural, optical and physicochemical properties. The synthesized nanocomposite exhibited distinctive absorption spectra and precise alignment of diffraction peaks, confirming the successful incorporation of yttrium ions into the cerium oxide lattice. Electron microscopy images revealed well-dispersed yttrium particles within the ceria matrix, indicating uniform distribution and morphology. DLS and zeta potential analysis provided insights into the size distribution and stability of the nanocomposite. Furthermore, in vivo toxicity assessment using Drosophila melanogaster model revealed no significant toxicity of Ce:Y nanocomposite, as evidenced by survival assay and behavioral assays. Superoxide dismutase (SOD) activity and reactive oxygen species (ROS) levels were also evaluated, demonstrating no discernible nanoparticle-induced toxicity. Overall, this study highlights the potential applications of Ce:Y nanocomposite and underscores the importance of comprehensive toxicity evaluation in nanomaterial development.