TOTAL PHENOLIC, FLAVONOID CONTENTS AND ANTIOXIDANT ACTIVITY OF STANDARDIZED EXTRACT OF GAGATAN HARIMAU LEAVES (VITIS GRACILIS BL)

Objective: The purpose of this research was to standardize the specific and nonspecific simplicia of Gagatan Harimau leaf, determine the total phenolic and total flavonoid contents of Gagatan Harimau leaf ethanol extract, and evaluate the antioxidant activity of Gagatan Harimau leaf ethanol extract. Methods: Total phenolic content was determined using the Folin-Ciocalteu method and gallic acid as standard comparison. Total flavonoid content using the aluminum chloride method with standard quercetin as a comparison. Antioxidant activity was analyzed using the DPPH method by measuring synthetic radical scavengers in polar solvents. Results: Standardization of specific and nonspecific simplicia results were pointed leaf tips and bases, pinnate bone placement, jagged and rough leaf margins, smooth hairy undersides, 14-24 cm long, width 6-11 cm, color green, characteristic odor and sour taste, Cu content<150 mg/l, bacterial contamination ≤ 10.000 colonies/g. The total phenolic and flavonoid content of the ethanol extract of Gagatan Harimau showed 207.6695±0.2056 mg GAE/g and 23.2883± 0.0556 mg QE/g. Antioxidant activity with a concentration of 10; 20; 30; 40; 50 µg/ml gives an IC50 value of 34.79 µg/ml. Conclusion: Gagatan Harimau leaves have a good antioxidant activity, so they can be used as a source of natural antioxidants.