Miniaturized protein profiling permits targeted signaling pathway analysis in individual circulating tumor cells to improve personalized treatment
The ZeptoCTC workflow represents a valuable tool in single cell cancer research, crucial for personalized medicine. It permits detailed analysis of key proteins and their activation status of targeted, cancer-driven signaling pathways in single cell samples, aiding in understanding tumor response, progression, and treatment efficacy beyond bulk analysis. The method significantly advances clinical investigations in cancer, improving treatment precision and effectiveness. The workflow will be applicable to protein analysis on other types of single cells like relevant in stem cell, neuropathology and hemopoietic cell research.
Authors
- Nadia Stamm
- Hans Neubauer (ORCID: https://orcid.org/0000-0002-3467-4105)
- Meike Beer
- Anna Abramova (ORCID: https://orcid.org/0009-0004-4660-8449)
- Mahdi Rivandi (ORCID: https://orcid.org/0000-0002-4554-8868)
- Jens Eberhardt
- Liwen Yang (ORCID: https://orcid.org/0000-0001-5638-1488)
- André Franken (ORCID: https://orcid.org/0000-0002-3508-5601)
- Berthold Gierke
- Dieter Niederacher (ORCID: https://orcid.org/0000-0001-6231-9226)
- Michael Pawlak (ORCID: https://orcid.org/0000-0002-6094-7285)
- Tanja Fehm (ORCID: https://orcid.org/0000-0002-4926-0108)
Institutions
- Düsseldorf University Hospital (DE)
- Integrated Oncology (United States) (US)
- Natural and Medical Sciences Institute (DE)
- Sartorius (Germany) (DE)
- Heinrich Heine University Düsseldorf (DE)
Publication Details
- Journal
- Journal of Translational Medicine
- Published
- 2024-09-20
- DOI
- https://doi.org/10.1186/s12967-024-05616-7
- Citations
- 3
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.86
Funders
- Deutsche Krebshilfe
- Servier