A Smartphone-Mediated “All-In-One” Biosensing Chip for Visual and Value-Assisted Detection
A smartphone-mediated self-powered biosensor is fabricated for miRNA-141 detection based on the CRISPR/Cas12a cross-cutting technique and a highly efficient nanozyme. As a novel nanozyme and a signal-amplified coreaction accelerator, the AuPtPd@GDY nanozyme exhibits an excellent ability to catalyze cascade color reactions and high conductivity to enhance the electrochemical signal for miRNA-141 assays. After CRISPR/Cas12a cross-cutting of S2-glucose oxidase (S2-GOD), the electrochemical signal is weakened, and miRNA-141 is detected by monitoring the decrease in the signal. On the other hand, a cascade reaction among glucose, H<sub>2</sub>O<sub>2</sub>, and TMB is catalyzed by GOD and AuPtPd@GDY, respectively, resulting in a color change of the solution, which senses miRNA-141. The self-powered biosensor enables value-assisted and visual detection of miRNA-141 with limits of detection of 3.1 and 15 aM, respectively. Based on the dual-modal self-powered sensing system, a smartphone-mediated "all-in-one" biosensing chip is designed to achieve the real-time and intelligent monitoring of miRNA-141. This work provides a new approach to design multifunctional biosensors to realize the visualization and portable detection of tumor biomarkers.
Authors
- Jing Xu (ORCID: https://orcid.org/0000-0001-8084-5609)
- Weihong Tan (ORCID: https://orcid.org/0000-0002-8066-1524)
- Futing Wang (ORCID: https://orcid.org/0000-0001-7306-5495)
- Yujin Li (ORCID: https://orcid.org/0000-0003-1103-3273)
- Hongfen Yang (ORCID: https://orcid.org/0000-0001-8367-4104)
- Ren Cai (ORCID: https://orcid.org/0000-0003-2583-7133)
- Ke‐Jing Huang (ORCID: https://orcid.org/0000-0002-9332-0497)
Institutions
- Xinyang Normal University (CN)
- Minzu University of China (CN)
- Hunan University (CN)
- Shanghai Jiao Tong University (CN)
- Renji Hospital (CN)
- Zhejiang Cancer Hospital (CN)
- Cancer Hospital of Chinese Academy of Medical Sciences (CN)
- University of Chinese Academy of Sciences (CN)
- University of South China (CN)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2024-09-20
- DOI
- https://doi.org/10.1021/acs.analchem.4c03854
- Citations
- 22
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 5.14
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Hunan Province
- Education Department of Henan Province
- Henan Provincial Science and Technology Research Project