Protocol for formation, staining, and imaging of 3D breast cancer models using MicroTissues mold systems
The use of 3D cancer models to study therapy response has a great advantage over conventional 2D cell culture. Here, we present an optimized protocol for breast cancer spheroid and rod-shaped microtissue formation using MicroTissues mold systems. We describe steps for cast formation and treating the 3D models with DNA-damaging agents. We then detail procedures for analyzing the 3D models by whole-mount immunostaining and confocal imaging of fixed samples or with the use of live-cell reporters.
Authors
- Maayke M.P. Kuijten
- Ben Haspels (ORCID: https://orcid.org/0000-0002-5802-9768)
Institutions
- Erasmus MC Cancer Institute (NL)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-09-20
- DOI
- https://doi.org/10.1016/j.xpro.2024.103250
- Citations
- 5
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.92
Funders
- Erasmus Medisch Centrum
- KWF Kankerbestrijding
- Oncode Institute