Metabologenomics-Inspired Discovery and Combinatorial Biosynthesis-Based Diversification of Fungal <i>O</i>-Glycosylated Depsides
Through metabologenomics mining, we prioritized <i>Exophiala xenobiotica</i> SDU 039, a deep-sea sediment-derived fungus producing <i>O</i>-glycosylated depsides (<b>1</b>-<b>9</b>), including seven new species with varying aliphatic chains. Heterologous expression validated the <i>exo</i> gene cluster, and <i>in vitro</i> enzyme assays elucidated the function of glycosyltransferase ExoC. The chemical diversity of <i>O</i>-glycosylated depsides is expanded by combinatorial biosynthesis using homologues depside biosynthetic genes and <i>in vitro</i> transformation with ExoC and different sugars as substrate.
Authors
- Cunlei Cai
- Jianhua Ju (ORCID: https://orcid.org/0000-0001-7712-8027)
- Zhuo Shang (ORCID: https://orcid.org/0000-0002-5755-2629)
- Yang Hu (ORCID: https://orcid.org/0000-0002-9646-5755)
- Kunlong Li (ORCID: https://orcid.org/0000-0002-1474-3097)
- Hongjie Zhu (ORCID: https://orcid.org/0000-0002-2604-033X)
- Jiafan Yang (ORCID: https://orcid.org/0000-0001-5703-0890)
- Yingying Chen (ORCID: https://orcid.org/0000-0001-6005-3516)
- Li Feng (ORCID: https://orcid.org/0000-0002-8692-7645)
- Ming Peng (ORCID: https://orcid.org/0009-0009-9710-639X)
Institutions
- Shandong University (CN)
- Chinese Academy of Sciences (CN)
- Institute of Oceanology (CN)
- South China Sea Institute Of Oceanology (CN)
Publication Details
- Journal
- Organic Letters
- Published
- 2024-09-20
- DOI
- https://doi.org/10.1021/acs.orglett.4c03024
- Citations
- 7
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 3.13
Funders
- National Natural Science Foundation of China
- National Key Research and Development Program of China