Involvement of Cav3.2 T-type Ca2+ channels and cystathionine-β-synthase in colitis-related visceral hypersensitivity in mice
We tested the hypothesis that Ca<sub>v</sub>3.2 T-type Ca<sup>2+</sup> channels, which can be rebooted by sulfides from Zn<sup>2+</sup> inhibition under physiological conditions, and sulfide-generating enzymes including cystathionine-β-synthase (CBS) would participate in the colitis-related visceral pain in mice treated with 2,4,6-trinitrobenzene sulfonic acid (TNBS). The visceral hypersensitivity following TNBS-induced colitis was abolished by an inhibitor or genetic deletion of Ca<sub>v</sub>3.2 and by a CBS inhibitor, and accompanied by CBS upregulation in the colon. Our data thus suggest that the enhanced activity of Ca<sub>v</sub>3.2 brought about by sulfides generated by upregulated CBS is involved in the colitis-related visceral hypersensitivity.
Authors
- Naoki Toyooka (ORCID: https://orcid.org/0000-0002-9568-1528)
- Yuriko Iba
- Fumiko Sekiguchi (ORCID: https://orcid.org/0000-0002-2785-0289)
- Atsushi Kawase
- Atsufumi Kawabata (ORCID: https://orcid.org/0000-0002-3163-807X)
- Takuya Okada (ORCID: https://orcid.org/0000-0003-2228-7711)
- Yoshihito Kasanami
- Maho Tsubota
- M. HONDA
- T Hatakeyama
Institutions
- University of Toyama (JP)
- Kindai University (JP)
Publication Details
- Journal
- Journal of Pharmacological Sciences
- Published
- 2024-09-21
- DOI
- https://doi.org/10.1016/j.jphs.2024.09.003
- Primary Topic
- Sulfur Compounds in Biology
- Type
- article
- Field-Weighted Citation Impact
- 0.00