Assemblable 3D biomimetic microenvironment for hMSC osteogenic differentiation
Adequate simulation mimicking a tissue's native environment is one of the elemental premises in tissue engineering. Although various attempts have been made to induce human mesenchymal stem cells (hMSC) into an osteogenic pathway, they are still far from widespread clinical application. Most strategies focus primarily on providing a specific type of cue, inadequately replicating the complexity of the bone microenvironment. An alternative multifunctional platform for hMSC osteogenic differentiation has been produced. It is based on poly(vinylidene fluoride) (PVDF) and cobalt ferrites magnetoelectric microspheres, functionalized with collagen and gelatin, and packed in a 3D arrangement. This platform is capable of performing mechanical stimulation of piezoelectric PVDF, mimicking the bones electromechanical biophysical cues. Surface functionalization with extracellular matrix biomolecules and osteogenic medium complete this all-round approach. hMSC were cultured in osteogenic inducing conditions and tested for proliferation, surface biomarkers, and gene expression to evaluate their osteogenic commitment.
Authors
- Clarisse Ribeiro (ORCID: https://orcid.org/0000-0002-9120-4847)
- Amparo Sempere (ORCID: https://orcid.org/0000-0001-6727-3343)
- Senentxu Lanceros-Méndez (ORCID: https://orcid.org/0000-0001-6791-7620)
- Luís Amaro Martins (ORCID: https://orcid.org/0000-0001-8648-6459)
- Joaquín Ródenas‐Rochina (ORCID: https://orcid.org/0000-0001-5764-207X)
- José Luís Gómez Ribelles (ORCID: https://orcid.org/0000-0001-9099-0885)
- Lourdes Cordón (ORCID: https://orcid.org/0000-0002-8808-3423)
- Nadia García-Parra (ORCID: https://orcid.org/0009-0007-7530-207X)
Institutions
- Ikerbasque (ES)
- Instituto de Salud Carlos III (ES)
- Basque Center for Materials, Applications and Nanostructures (ES)
- Hospital Universitari i Politècnic La Fe (ES)
- Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (ES)
- Centro de Investigación Biomédica en Red de Cáncer (ES)
- Instituto de Investigación Sanitaria La Fe (ES)
- Universitat Politècnica de València (ES)
- University of Minho (PT)
Publication Details
- Journal
- Biomedical Materials
- Published
- 2024-09-20
- DOI
- https://doi.org/10.1088/1748-605x/ad7dc4
- Citations
- 1
- Primary Topic
- Bone Tissue Engineering Materials
- Type
- article
- Field-Weighted Citation Impact
- 0.18
Funders
- Ministerio de Ciencia e Innovación
- Fundação para a Ciência e a Tecnologia