Green synthesis of Titanium dioxide nanoparticle using combination of plants and its antimicrobial study with their characterization

Abstract The characteristics and biological qualities of the nanomaterial rely heavily on how it was made. Green nanoparticle production has been developed to minimize pollution, cut expenses, and enhance safety for both human health and the environment by reducing metal ions using plant extracts as opposed to industrial chemical agents. The goal of the current work is to synthesize titanium dioxide nanoparticles in an environmentally friendly manner by using an extract from the combined shells of Stone apples ( Aegle marmelos ) and Wood apples ( Limonia acidissima ). Titanium dioxide nanoparticle formation was verified using various characterization techniques. Well diffusion was used to measure the antimicrobial activity. The fungal strains that were employed were Aspergillus Niger, Candida Albicans , and Aspergillus Flavus . All fungul strains were successfully inhibited by both the crudely prepared extract and the biosynthesized titanium dioxide nanoparticles; however, the biosynthesized titanium dioxide nanoparticles exhibited a high zone of inhibition ranging from 25 to 30 mm, while the crudely prepared extract had a low zone of inhibition ranging from 13 to 19 mm. A moderately sized zone of inhibition was observed in both the crude produced extract and the biosynthesized Titanium dioxide nanoparticles at a dilution of 100 μg ml −1 . Lower dilutions demonstrated less noticeable inhibition. Overall, these results showed that treatment with biosynthesized titanium dioxide nanoparticles significantly slowed the growth of many microorganisms.