Replacing sperm with genotyped haploid androgenetic blastomeres to generate cattle with predetermined paternal genomes
Haploid morula-stage embryonic cells derived from a single sperm can be genotyped and injected into activated oocytes to reconstruct diploid zygotes that develop both in vitro into blastocysts and in vivo into viable post-implantation bovine conceptuses with predetermined paternal genomes.
Authors
- Luis Águila (ORCID: https://orcid.org/0000-0001-9713-4248)
- Jacinthe Therrien (ORCID: https://orcid.org/0000-0002-4364-8136)
- Rafael Vilar Sampaio (ORCID: https://orcid.org/0000-0002-8227-8510)
- Rémi Labrecque (ORCID: https://orcid.org/0000-0003-2129-7336)
- Amélie Tremblay (ORCID: https://orcid.org/0000-0002-7735-3539)
- Lawrence C. Smith (ORCID: https://orcid.org/0000-0001-8766-0148)
- Ricardo Perecin Nociti (ORCID: https://orcid.org/0000-0002-0831-5264)
- Patrick Blondin
- Gabrielle Marras
Institutions
- Universidad de La Frontera (CL)
- Cegep de Saint Hyacinthe (CA)
- Biotechnology Research Center (IR)
- L'Alliance Boviteq (CA)
- Université de Montréal (CA)
Publication Details
- Journal
- Biology of Reproduction
- Published
- 2024-09-20
- DOI
- https://doi.org/10.1093/biolre/ioae135
- Primary Topic
- Reproductive Biology and Fertility
- Type
- article
- Field-Weighted Citation Impact
- 0.00