nQuant Enables Precise Quantitative N-Glycomics
N-glycosylation is a highly heterogeneous post-translational modification that modulates protein function. Defects in N-glycosylation are directly linked to various human diseases. Despite the importance of quantifying N-glycans with high precision, existing glycoinformatics tools are limited. Here, we developed nQuant, a glycoinformatics tool that enables label-free and isotopic labeling quantification of N-glycomics data obtained via LC-MS/MS, ensuring a low false quantitation rate. Using the label-free quantification module, we profiled the N-glycans released from purified glycoproteins and HEK293 cells as well as the dynamic changes of N-glycosylation during mouse corpus callosum development. Through the isotopic labeling quantification module, we revealed the dynamic changes of N-glycans in acute promyelocytic leukemia cells after all-trans retinoic acid treatment. Taken together, we demonstrate that nQuant enables fast and precise quantitative N-glycomics.
Authors
- Jiankai Luo (ORCID: https://orcid.org/0000-0001-5169-3095)
- Xihai Li (ORCID: https://orcid.org/0000-0001-8348-8256)
- Yudong Guan (ORCID: https://orcid.org/0000-0003-1350-5238)
- Hartmut Schlüter (ORCID: https://orcid.org/0000-0002-9358-7036)
- Shanshan Zhao
- Chunjin Fu (ORCID: https://orcid.org/0000-0002-8286-777X)
- Chengchao Xu (ORCID: https://orcid.org/0000-0003-4558-7581)
- Jigang Wang (ORCID: https://orcid.org/0000-0002-0575-0105)
- Lingyun Dai (ORCID: https://orcid.org/0000-0001-9386-5967)
- Fan Yang (ORCID: https://orcid.org/0000-0001-6306-4501)
- Junzhe Zhang (ORCID: https://orcid.org/0000-0003-3471-037X)
Institutions
- Fujian University of Traditional Chinese Medicine (CN)
- Universität Hamburg (DE)
- Henan University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Southern University of Science and Technology (CN)
- University Medical Center Hamburg-Eppendorf (DE)
- University of Rostock (DE)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2024-09-20
- DOI
- https://doi.org/10.1021/acs.analchem.4c01153
- Citations
- 5
- Primary Topic
- Glycosylation and Glycoproteins Research
- Type
- article
- Field-Weighted Citation Impact
- 1.17
Funders
- National Natural Science Foundation of China
- National Key Research and Development Program of China