Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation
A human induced pluripotent stem cell (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC. The iPSC line expresses pluripotency markers, displays a normal karyotype, and is able to differentiate into three germ layers in vitro. This iPSC line represents a valuable cell model for NF1 in humans.
Authors
- Aihua Ji
- Hongmei Xin (ORCID: https://orcid.org/0000-0003-4787-8198)
- Yixiao Li (ORCID: https://orcid.org/0009-0000-6551-6372)
- Kaihui Zhang (ORCID: https://orcid.org/0000-0003-4799-9133)
- Meili Fan
Institutions
- Shandong University (CN)
- Shandong Provincial Hospital (CN)
- Qilu Hospital of Shandong University (CN)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2024-09-20
- DOI
- https://doi.org/10.1016/j.scr.2024.103568
- Citations
- 1
- Primary Topic
- Neurofibromatosis and Schwannoma Cases
- Type
- article
- Field-Weighted Citation Impact
- 0.37
Funders
- Center for Depression Research and Clinical Care, University of Texas Southwestern Medical Center