Research On The Cooling Treatment Plans For An Animal Model Of Exertional Heat Stroke

Corresponding author: Xiuzhen Liu. The animal model of exertional heat stroke(EHS) plays an important role in the study of cooling first aid for EHS. However, the plan and effectiveness of cooling treatment for the EHS animal model is still unclear. PURPOSE: To determine the plan and effectiveness of cooling treatment for the EHS animal model with cold water immersion(CWI). METHODS: There are five groups of male SD rats, including the treatment group with 2 °C CWI after EHS(CWI2,n = 6), the treatment group with 9 °C CWI after EHS(CWI9,n = 6), the treatment group with 19 °C CWI after EHS(CWI19,n = 7), the treatment effect group with CWI after EHS(EEHS,n = 8) and the natural rest group after EHS(REHS,n = 9). All rats were used to establish the EHS model by simulating marathon to raise their core temperature to about 42 °C. After determining the right cooling treatment plan by studying the first three groups, we applied the plan to the last two groups to explore the effectiveness of cooling treatment.The tester used a rat anal thermometer (JNT-200 model from Beijing Bobei Technology Co., Ltd.) to measure the rectal temperature as the core temperature .The Mann-Whitney U test of Nonparametric testing is used for statistical analysis. RESULTS: More than half of EHS rats died after being treated with CWI, while All 6 rats immersed in cold water at 19 °C for 5 min survived.Thus, we choose the cooling treatment plan of 19 °C CWI for 5 min to study the cooling effect. At the beginning of EHS, there was no significant difference in core temperature between the EEHS group and the REHS group(EEHS vs. REHS)(T-rec: 41.73 ± 0.83 vs. 41.73 ± 0.54 °C, p > 0.05). After cooling treatment, there were significant differences in core temperature and cooling rate between the EEHS group and the REHS group(EEHS vs. REHS)(T-rec: 35.95 ± 1.19 vs. 39.68 ± 0.61 °C, p < 0.01; R-cool: 1.15 ± 0.24 vs. 0.41 ± 0.15 °C/min, p < 0.01). CONCLUSIONS: The cooling treatment plan of cold water immersion at 19 °C for 5 min is appropriate,which can ensure both the survival rate and the cooling effect of EHS rats. Thus it can be further used for the study of the pathophysiological mechanisms of cooling treatment for exertional heat stroke. The research of this thesis has sponsored by the key fund project of Scientific Research Compilation Plan Project in Anhui Province in 2022(Project Number: 2022AH051031). The research of this thesis has sponsored by the key fund project of Scientific Research Compilation Plan Project in Anhui Province in 2022(Project Number: 2022AH051031).