Preliminary Evidence Of The Effects Of Extenuating Exercise On Liver Biomarkers In Hyperlipidemic Mice

Preliminary evidence of the effects of extenuating exercise on liver biomarkers in hyperlipidemic mice Wagner Luiz do Prado1; Ellem Eduarda Pinheiro dos Santos3; Lila Missae Oyama2; Felipe Avila2; Rafael Ishihara Figueirôa3; Rafael Herling Lambertucci3; João Paulo Botero31 California State University San Bernardino, California, USA.2 Federal University of São Paulo (UNIFESP), São Paulo, Brazil.3 Federal University of São Paulo (UNIFESP) Campus Baixada Santista, Santos, São Paulo, Brazil. Oxidative stress (OS) plays a role in the progression of liver disorders. Exercise is recognized as an effective and safe strategy for managing such disorders. It is plausible that acute bouts of exercise may lead to undesirable short-term liver responses associated with OS. However, these responses have been inadequately characterized in liver disorders, such as non-alcoholic liver disease. Purpose: This study aims to examine the acute effects of strenuous exercise on OS biomarkers in liver tissue. Methods: Thirty-one male C57BL/6 mice were randomly assigned to either a normal diet (control, N = 16) or a hyperlipidic diet (N = 15) for 10 weeks. Following the dietary protocol, mice were divided into sedentary (n = 7) or exercise conditions (n = 24 - a single bout of exercise on a treadmill until exhaustion). The exercised animals were euthanized at 6 h, 24 h, or 48 h after the exercise session. Thiobarbituric acid reactive substance (TBARS) and nitrite levels were assessed in liver tissue using standard methods. Results: table 1. Conclusion: The impact of a single bout of strenuous exercise on oxidative stress in the liver tissue of mice may be influenced by diet. These findings provide preliminary evidence that exercise could induce varying responses in liver tissue oxidative stress among animals subjected to a hyperlipidic diet. Table 1 - -TBARS and nitrite concentration in liver tissue in mice submitted to extenuating exercise, according to diet. Diet Time N Mean ± SD p-valueDiet Time Diet x Time TBARS Liver Control No exercise 4 0.337 ± 0.0865 6 h after 4 0.842 ± 0.2221 0.241 0.782 < 0.001 24 h after 4 1.217 ± 0.6203 48 h after 4 1.397 ± 0.5846 Hyperlipidic No exercise 3 1.104 ± 0.3081 6 h after 4 0.851 ± 0.7037 24 h after 4 0.627 ± 0.2892 48 h after 4 0.486 ± 0.1488 Nitrite Liver Control No exercise 4 1.771 ± 0.7037 0.131 0.910 < 0.001 6 h after 4 3.648 ± 16.789 24 h after 4 3.424 ± 0.9931 48 h after 4 3.978 ± 23.284 Hyperlipidic No exercise 3 5.934 ± 13.134 6 h after 4 3.543 ± 0.8110 24 h after 4 3.191 ± 0.8329 48 h after 4 3.194 ± 10.502