Structural characterization and prebiotic activity of oligo-galacturonic acids from Osmunda japonica Thunb

An oligosaccharide, WOJP-AOS-b, with a molecular size of 1.8 kDa, was obtained through enzymatic hydrolysis of Osmunda japonica Thunb polysaccharides (WOJP-A). The structural characteristics of WOJP-AOS-b were elucidated using Fourier transform-infrared spectroscopy (FT-IR), Ultraviolet (UV) spectroscopy, Nuclear magnetic resonance (NMR) spectroscopy, and Eelectrospray ionization tandem mass spectrometry (ESI-MS/MS). Structural analysis revealed that galacturonic acid (Gal p A) was the predominant monosaccharide component of WOJP-AOS-b, accounting for 90.91% of the total composition. FT-IR and NMR analyses confirmed that WOJP-AOS-b possesses a linear structure comprised of α-1,4-linked Gal p A residues. MS/MS results indicated that WOJP-AOS-b consists of α-1,4-linked oligo-galacturonic acids with degrees of polymerization ranging from 2 to 6 (DP 2–6). Moreover, in vitro probiotic activity assays demonstrated that WOJP-AOS-b exerted beneficial effects on intestinal microbiota, particularly Bifidobacterium adolescentis ATCC 15703 and Bifidobacterium breve 689b. WOJP-AOS-b enhanced the optical density, short-chain fatty acid (SCFA) content, and lactic acid content in the culture medium. Furthermore, 16S rRNA analysis suggested that WOJP-AOS-b exhibits potential for treating obesity and inhibiting pathogenic bacteria, indicating its promising applications as a prebiotic. • An oligosaccharide WOJP-AOS-b was prepared from Osmunda japonica Thunb. • WOJP-AOS-b is an α-1,4-oligogalacturonic acid, with a degree of polymerization 2–6. • WOJP-AOS-b exhibited beneficial effects and had the potential as a prebiotic.