Endogenous viral elements are targeted by <scp>RNA</scp> silencing pathways in banana
Endogenous banana streak virus (eBSV) integrants derived from three distinct species, present in Musa balbisiana (B) but not Musa acuminata (A) banana genomes are able to reconstitute functional episomal viruses causing banana streak disease in interspecific triploid AAB banana hybrids but not in the diploid (BB) parent line, which harbours identical eBSV loci. Here, we investigated the regulation of these eBSV. In-depth characterization of siRNAs, transcripts and methylation derived from eBSV using Illumina and bisulfite sequencing were carried out on eBSV-free Musa acuminata AAA plants and BB or AAB banana plants with eBSV. eBSV loci produce low-abundance transcripts covering most of the viral sequence and generate predominantly 24-nt siRNAs. siRNA accumulation is restricted to duplicated and inverted viral sequences present in eBSV. Both siRNA-accumulating and nonaccumulating sequences of eBSV in BB plants are heavily methylated in all three CG, CHG and CHH contexts. Our data suggest that eBSVs are controlled at the epigenetic level in BB diploids. This regulation not only prevents their awakening and systemic infection of the plant but is also probably involved in the inherent resistance of the BB plants to mealybug-transmitted viral infection. These findings are thus of relevance to other plant resources hosting integrated viruses.
Authors
- Frédéric Salmon (ORCID: https://orcid.org/0000-0002-7529-4184)
- Jean-Marie Eric Delos
- Jonathan Séguin (ORCID: https://orcid.org/0000-0002-3676-0437)
- Nathalie Laboureau (ORCID: https://orcid.org/0000-0002-0105-3919)
- Marie‐Line Iskra‐Caruana (ORCID: https://orcid.org/0000-0003-4486-2449)
- Rajendran Rajeswaran (ORCID: https://orcid.org/0000-0002-4842-1256)
- Sébastien Ravel (ORCID: https://orcid.org/0000-0001-6663-782X)
- Pierre‐Olivier Duroy (ORCID: https://orcid.org/0000-0002-4045-5240)
- Mikhail M. Pooggin (ORCID: https://orcid.org/0000-0003-2308-393X)
- Matthieu Chabannes (ORCID: https://orcid.org/0000-0001-5754-5982)
Institutions
- Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR)
- University of Basel (CH)
- Université de Montpellier (FR)
- Institut Agro Montpellier (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Amélioration Génétique et Adaptation des Plantes méditerranéennes et tropicales (FR)
- Institut Agro Montpelier (FR)
- Services déconcentrés d'appui à la recherche Occitanie-Montpellier (FR)
- Institut de Recherche pour le Développement (FR)
- Plant Health Institute de Montpellier (FR)
- L'Institut Agro (FR)
Publication Details
- Journal
- New Phytologist
- Published
- 2024-09-18
- DOI
- https://doi.org/10.1111/nph.20112
- Citations
- 2
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.20
Funders
- European Cooperation in Science and Technology
- Centre de Coopération Internationale en Recherche Agronomique pour le Développement