Effects of Sterilization on Cellobiose Dehydrogenase and Glucose Oxidase Based Glucose Biosensors
Abstract Research on implantable glucose biosensors is driven by the need for innovative medical devices for continuous glucose monitoring in patients with diabetes mellitus. However, biosensor sterilization is a step that is widely omitted during the process of innovation. To compare the effects of gamma irradiation and chemical treatment with ethylene oxide (carbon microarray electrodes are fabricated, functionalized with glucose oxidizing enzymes (cellobiose dehydrogenase CDH or glucose oxidase GOx), and coated with a specifically designed zwitterionic polymer prior to the sterilization step. Cyclic voltammetry in the presence of 100 m m glucose of the biosensors before and after sterilization shows that gamma irradiation with a low radiation rate (25 kGy, 260 Gy h −1 ) does not induce a sensor performance loss, unlike the EtO treatment. In addition, no cytotoxic by‐products are released after gamma sterilization. Based on these results obtained with both glucose oxidizing enzymes (CDH and GOx), gamma irradiation of the glucose biosensors with a low dose rate is preferable to exposure to EtO for biosensor terminal sterilization.
Authors
- Kavita Jayakumar (ORCID: https://orcid.org/0000-0001-6354-2315)
- Richard Bennett (ORCID: https://orcid.org/0000-0002-3777-3467)
- Thomas M. B. Reichhart (ORCID: https://orcid.org/0000-0002-0538-108X)
- Andrew R. Mount (ORCID: https://orcid.org/0000-0003-1237-8206)
- Sébastien Gounel (ORCID: https://orcid.org/0000-0002-7602-9657)
- Wolfgang W. Schuhmann (ORCID: https://orcid.org/0000-0003-2916-5223)
- Nicolas Mano (ORCID: https://orcid.org/0000-0001-7084-9323)
- Roland Ludwig (ORCID: https://orcid.org/0000-0002-5058-5874)
- Claudine Boiziau (ORCID: https://orcid.org/0000-0002-0475-2571)
- Alfons K. G. Felice (ORCID: https://orcid.org/0000-0002-3842-1471)
- Dónal Leech (ORCID: https://orcid.org/0000-0001-7844-1306)
- Anna Lielpētere (ORCID: https://orcid.org/0000-0002-4727-0935)
- Arvind Rathore
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université de Bordeaux (FR)
- Inserm (FR)
- Ollscoil na Gaillimhe – University of Galway (IE)
- Bordeaux Population Health (FR)
- Center for Research in Psychopathology and Clinical Psychology (FR)
- Centre de recherche Paul Pascal (FR)
- Laboratory for the Bioengineering of Tissues (FR)
- Ruhr University Bochum (DE)
- BOKU University (AT)
- University of Edinburgh (GB)
Publication Details
- Journal
- Advanced Sensor Research
- Published
- 2024-09-18
- DOI
- https://doi.org/10.1002/adsr.202400056
- Citations
- 2
- Primary Topic
- Electrochemical sensors and biosensors
- Type
- article
- Field-Weighted Citation Impact
- 0.42
Funders
- European Commission
- H2020 Marie Skłodowska-Curie Actions