Performance of an in-house multiplex PCR assay for HIV-1 drug resistance testing – A cheaper alternative
The in-house multiplex PCR assay demonstrated satisfactory performance and higher sensitivity for IN fragment amplification. This could be a cost-effective method for HIV-1 drug resistance testing as both PR-RT and IN fragments are successfully amplified in one reaction in most samples.
Authors
- Raquel Viana (ORCID: https://orcid.org/0000-0002-9640-1209)
- Paballo Nkone
- Allison Glass
- Shayne Loubser (ORCID: https://orcid.org/0000-0001-9718-4085)
- Caroline T. Tiemessen (ORCID: https://orcid.org/0000-0002-0991-1690)
- Simnikiwe H. Mayaphi
- Tumelo L Fortuin
- Keitumetse Moeng
Institutions
- Tshwane University of Technology (ZA)
- University of the Witwatersrand (ZA)
- Sefako Makgatho Health Sciences University (ZA)
- Lancet Laboratories (ZA)
- University of Pretoria (ZA)
Publication Details
- Journal
- Journal of Virological Methods
- Published
- 2024-09-19
- DOI
- https://doi.org/10.1016/j.jviromet.2024.115034
- Citations
- 1
- Primary Topic
- HIV Research and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.29
Funders
- National Research Foundation
- Poliomyelitis Research Foundation
- University of Pretoria
- National Health Laboratory Service