Extracellular vesicle-mediated trafficking of molecular cues during human brain development
Cellular crosstalk is an essential process influenced by numerous factors, including secreted vesicles that transfer nucleic acids, lipids, and proteins between cells. Extracellular vesicles (EVs) have been the center of many studies focusing on neurodegenerative disorders, but whether EVs display cell-type-specific features for cellular crosstalk during neurodevelopment is unknown. Here, using human-induced pluripotent stem cell-derived cerebral organoids, neural progenitors, neurons, and astrocytes, we identify heterogeneity in EV protein content and dynamics in a cell-type-specific and time-dependent manner. Our results support the trafficking of key molecules via EVs in neurodevelopment, such as the transcription factor YAP1, and their localization to differing cell compartments depending on the EV recipient cell type. This study sheds new light on the biology of EVs during human brain development.
Authors
- Mariano Gonzalez Pisfil (ORCID: https://orcid.org/0000-0002-5008-8763)
- Pavel Kielkowski (ORCID: https://orcid.org/0000-0003-4910-6263)
- Natalia Baumann (ORCID: https://orcid.org/0000-0003-4518-2103)
- Michael W. Pfaffl (ORCID: https://orcid.org/0000-0002-3192-1019)
- Benno Pütz (ORCID: https://orcid.org/0000-0002-2208-209X)
- Sylvain Moser (ORCID: https://orcid.org/0000-0003-4261-5866)
- Rossella Di Giaimo (ORCID: https://orcid.org/0000-0003-3604-3697)
- Filippo M. Cernilogar (ORCID: https://orcid.org/0000-0003-2814-3436)
- Andrea Carolina Pérez Forero (ORCID: https://orcid.org/0000-0002-7163-8365)
- Fabrizia Pipicelli (ORCID: https://orcid.org/0000-0001-8641-8317)
- Silvia Cappello (ORCID: https://orcid.org/0000-0003-1553-6775)
- Christian Grätz (ORCID: https://orcid.org/0000-0002-9356-7940)
- Giuseppina Maccarrone
Institutions
- University of Geneva (CH)
- Centre of Experimental Medicine of the Slovak Academy of Sciences (SK)
- Max Planck Institute of Psychiatry (DE)
- Technical University of Munich (DE)
- Ludwig-Maximilians-Universität München (DE)
Publication Details
- Journal
- Cell Reports
- Published
- 2024-09-19
- DOI
- https://doi.org/10.1016/j.celrep.2024.114755
- Citations
- 19
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 4.44
Funders
- E-Rare
- European Commission
- Deutsche Forschungsgemeinschaft
- Fritz Thyssen Stiftung
- Ludwig-Maximilians-Universität München
- Ministero degli Affari Esteri e della Cooperazione Internazionale