The Antioxidant Properties of Salix gracilistyla Extract and Its Role in Shielding Cells from Oxidative Stress

This study aimed to explore the potential application of Salix gracilistyla leaf extract to the skin, evaluating its antioxidant effects and protective properties against oxidative stress using human-derived keratinocytes. The findings revealed that the extract exhibited significant DPPH radical scavenging activity with an IC50 value of 109.29± μg/mL, indicating a dose-dependent increase in scavenging activity. Furthermore, the extract contained notable levels of total polyphenols (203.13±3.25 μg/mL) and flavonoids (154.31±1.16 μg/mL). No cytotoxicity was observed in HaCaT cells at concentrations below 100 μg/mL. Additionally, the extract demonstrated dose-dependent protective effects against hydrogen peroxide-induced oxidative stress. These results imply that Salix gracilistyla leaf extract holds promise for antioxidant skincare applications, potentially mitigating harmful various factors that can damage the skin. Further research in this area is expected to deepen our understanding of its benefits and applications.